Predictions suggest that global potato production could exceed 400 Mt per year if diseases that reduce yields by ~25% could be controlled ( Agrios, 1997). In many parts of the world fungicide application is the only means to prevent disease. A conservative estimate of the chemical control costs and yield losses associated with late blight exceeds €6.7 Billion ( Haverkort et al., 2009). Previously resistant potato cultivars such as Lady Balfour and Stirling were susceptible to the 13-A2 lineage and are consequently no longer suitable for the organically grown potato market. infestans population a new clonal lineage referred to as 13-A2 or “blue 13” was first detected in 2004 and, upon its arrival in Great Britain, came to dominate the population within 3 years ( Cooke et al., 2012). infestans lineages have been observed that often impact negatively on crop production. In agricultural systems major population changes of P. Pests and pathogens represent a serious and continuing threat to potato production, and the most widespread and economically significant of these is late blight, caused by the oomycete pathogen Phytophthora infestans. Global potato production between 19 has shown an increase of 21% that is driven by a 48% rise of potato production in the developing world, where the growing area has increased alongside yield. Potato is the most important non-cereal food crop worldwide and is consumed by more than a billion people ( Birch et al., 2012). We provide evidence that existing germplasm collections are important sources of novel resistances and that “omic” technologies such as dRenSeq-based genomics and effector-omics are efficacious tools to rapidly explore the diversity within these collections. okadae accessions 7129, 7625, and 7629 contain functional Rpi-vnt1.1, they also carry a novel resistance gene. infestans isolates that have a broad spectrum of virulence demonstrated that, although S. okadae accession 3761 and a bulk of 20 susceptible progeny lacked sequence homology in the 5′ end compared to the functional Rpi-vnt1.1 gene. okadae accessions 7129, 7625, 3762, and a bulk of 20 resistant progeny confirmed the presence of full-length Rpi-vnt1.1 under stringent mapping conditions and corroborated allele mining results in the accessions 71 as well as Avr-vnt1 recognition in transient expression assays. Effector-omics, allele mining, and diagnostic RenSeq (dRenSeq) were utilized to investigate the nature of resistances in S. infestans isolates belonging to the genotype 13-A2 identified resistances in the species S. A screen of 126 wild diploid Solanum accessions from the Commonwealth Potato Collection (CPC) with P. The greatest threat to potato production world-wide is late blight, caused by the oomycete pathogen Phytophthora infestans. 2Information and Computational Sciences, The James Hutton Institute, Dundee, UK.1Cell and Molecular Sciences, The James Hutton Institute, Dundee, UK.Van Weymers 1 †, Katie Baker 2 †, Xinwei Chen 1 †, Brian Harrower 1, David E.
0 Comments
Leave a Reply. |